Molecular Biology Laboratory Methods

Welcome to our Molecular Biology Lab Methods section

This section is aimed as a guide to commonly used methods used in a plant biology lab. It includes general methods associated with the use of DNA, RNA and proteins. In the future it will include guides to more specif topics such as Gel shifts, use of databases and microarray filtering techniques.   

Running Agarose gels	Agrobacterium plant transformation
GUS reporter activity	Seed Sterilisation
Genomic DNA extraction	Bacterial cell transformation
Polymerase chain reaction	Plant RNA extraction
Radio-labelling probes	Northern Blotting
microarray analysis	EMS mutagenesis
Alkaline lysis minipreps	Agrobacterium miniprep
Denaturing RNA	Reverse transcription
Probe hybridisation